RARE-Seq - Random priming & Affinity capture of cell-free RNA fragments for Enrichment analysis by Sequencing

This is the supplemental website from the Alizadeh Lab and the Diehn Lab for the publication titled "An ultrasensitive method for detection of cell-free RNA" by Nesselbush, Luca, and Jeon, et al. This publication is currently under review.

RARE-Seq is a novel non-invasive cell-free RNA (cfRNA) sequencing method that incorporates end-to-end optimization from blood collection through bioinformatic analysis. The sequencing data generated by RARE-Seq enables simultaneous gene expression analyses (such as differential expression and gene set enrichment analyses) and variant calling directly from RNA. We demonstrate that his method can be used for multiple oncologic applications, including non-invasive cancer detection and genotyping, treatment resistance monitoring, tissue of origin (TOO) classification. Additionally, this method can be used in various clinical contexts beyond oncology, such as for detection of tissue injury associated with benign conditions or monitoring pharmacokinetic and pharmacodynamic responses to RNA therapies.

RARE-Seq project code can also be accessed on Github at https://github.com/RARESeq/